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Antimicrobial Peptides (AMPs)/Host Defense Peptides
Identification of Novel Antibacterial Peptides by Chemoinformatics and Machine Learning
Synergistic interaction between silver nanoparticles and membrane-permeabilizing antimicrobial peptides
Screening and characterization of surface-tethered cationic peptides for antimicrobial activity
Use of artificial intelligence in the design of small peptide antibiotics effective against a broad spectrum of highly antibiotic-resistant superbugs
Identification of novel host defense peptides and the absence of alpha-defensins in the bovine genome
Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances
Evaluating different descriptors for model design of antimicrobial peptides with enhanced activity toward P. aeruginosa
Using intrinsic X-ray absorption spectral differences to identify and map peptides and proteins
Use of luminescent bacteria for rapid screening and characterization of short cationic antimicrobial peptides synthesized on cellulose using peptide array technology

Antibacterial Host Defence Peptides of Bovine Origin

Sequence requirements and an optimization strategy for short antimicrobial peptides
High-throughput generation of small antibacterial peptides with improved activity

Inhibitor/Protease Interaction

Complete substitutional analysis of a sunflower trypsin inhibitor with different serine proteases.
Unraveling sub-site specificities of peptidic serine protease inhibitors by substitutional and structural analysis
Crystallization and preliminary x-ray analysis of complexes of porcine pancreatic elastase with two natural inhibitors
Design and characterization of a hybrid miniprotein that specifically inhibits porcine pancreatic elastase
Structure of a hybrid squash inhibitor in complex with porcine pancreatic elastase at 1.8 A resolution
Crystallization and preliminary X-ray analysis of the complex of porcine pancreatic elastase and a hybrid squash inhibitor
Characterizing and optimizing protease/peptide inhibitor interactions, a new application for spot synthesis
A novel method for characterizing protease/inhibitor interaction by using peptide synthesis on cellulose

SPOT Synthesis (Methods & Applications)

Screening and characterization of surface-tethered cationic peptides for antimicrobial activity
Use of artificial intelligence in the design of small peptide antibiotics effective against a broad spectrum of highly antibiotic-resistant superbugs
Use of luminescent bacteria for rapid screening and characterization of short cationic antimicrobial peptides synthesized on cellulose using peptide array technology
Peptide arrays on cellulose support: SPOT synthesis, a time and cost efficient method for synthesis of large numbers of peptides in a parallel and addressable fashion
Antibacterial Host Defence Peptides of Bovine Origin
 Cellulose-bound peptide arrays: preparation and applications
Sequence requirements and an optimization strategy for short antimicrobial peptides
Complete substitutional analysis of a sunflower trypsin inhibitor with different serine proteases
High-throughput generation of small antibacterial peptides with improved activity
Unraveling sub-site specificities of peptidic serine protease inhibitors by substitutional and structural analysis
Design and characterization of a hybrid miniprotein that specifically inhibits porcine pancreatic elastase
Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose
Characterizing and optimizing protease/peptide inhibitor interactions, a new application for spot synthesis
A novel method for characterizing protease/inhibitor interaction by using peptide synthesis on cellulose
Interaction of the capsid protein p24 (HIV-1) with sequence-derived peptides: influence on p24 dimerization

Methods:

Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances
Use of luminescent bacteria for rapid screening and characterization of short cationic antimicrobial peptides synthesized on cellulose using peptide array technology
Peptide arrays on cellulose support: SPOT synthesis, a time and cost efficient method for synthesis of large numbers of peptides in a parallel and addressable fashion
Cellulose-bound peptide arrays: preparation and applications
A novel method for characterizing protease/inhibitor interaction by using peptide synthesis on cellulose
High-throughput generation of small antibacterial peptides with improved activity

Reviews:

Antibacterial Host Defence Peptides of Bovine Origin
Cellulose-bound peptide arrays: preparation and applicationsCellulose-bound peptide arrays: preparation and applications
Unraveling sub-site specificities of peptidic serine protease inhibitors by substitutional and structural analysis

 

 

  • Antimicrobial Peptides (AMPs)/Host Defense Peptides

    Interpretable Features for the Activity Prediction of Short Antimicrobial Peptides Using Fuzzy Logic

    Mikut R, Hilpert K.

    Int J Pept Res Ther    . In press.
  • Identification of Novel Antibacterial Peptides by Chemoinformatics and Machine Learning

    Fjell CD, Jenssen H, Hilpert K; Cheung W, Panté N; Hancock REWW; Cherkasov A.

    J Med Chem    . In press.
  • Synergistic interaction between silver nanoparticles and membrane-permeabilizing antimicrobial peptides

    Ruden S, Hilpert K, Berditsch M, Wadhwani P, Ulrich AS.

    Antimicrob Agents Chemother.     In press.
  • Screening and characterization of surface-tethered cationic peptides for antimicrobial activity.

    Hilpert K    , Elliott M, Jenssen H, Kindrachuk J, Fjell CD, Körner J, Winkler DF, Weaver LL, Henklein P, Ulrich AS, Chiang SH, Farmer SW, Pante N, Volkmer R, Hancock REW.

    Chem Biol.     2009;16(1):58-69.
  • Use of artificial intelligence in the design of small peptide antibiotics effective against a broad spectrum of highly antibiotic-resistant superbugs.

    Hilpert K, Cherkasov A     (both authors contributed equally, order changed), Jenssen H, Fjell CD, Waldbrook M, Mullaly SC, Volkmer R, Hancock REW.

    ACS Chem Biol.     2009;4(1):65-74.

  • Identification of novel host defense peptides and the absence of alpha-defensins in the bovine genome.

    Fjell CD, Jenssen H, Fries P, Aich P, Griebel P, Hilpert K, Hancock REW, Cherkasov A.

    Proteins    . 2008;73(2):420-30.
  • Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances.

    Wiegand I, Hilpert K, Hancock REW.

    Nat Protoc    . 2008;3(2):163-75.

  • Evaluating different descriptors for model design of antimicrobial peptides with enhanced activity toward P. aeruginosa.

    Jenssen H, Lejon T, Hilpert K, Fjell CD, Cherkasov A, Hancock REW.

    Chem Biol Drug Des    . 2007;70(2):134-42.

  • Using intrinsic X-ray absorption spectral differences to identify and map peptides and proteins.

    Stewart-Ornstein J, Hitchcock AP, Hernández Cruz D, Henklein P, Overhage J, Hilpert K, Hale JD, Hancock REW.

    J Phys Chem     B. 2007;111(26):7691-9.

  • Use of luminescent bacteria for rapid screening and characterization of short cationic antimicrobial peptides synthesized on cellulose using peptide array technology.

    Hilpert K    , Hancock REW.

    Nat Protoc    . 2007;2(7):1652-60.

  • Antibacterial Host Defence Peptides of Bovine Origin

    Jenssen H, Hilpert K, Hancock REW.

    Journal Chimica Oggi/CHEMISTRY TODAY     2007;25(3):17-19.

  • Sequence requirements and an optimization strategy for short antimicrobial peptides

    Hilpert K    , Elliott MR, Volkmer-Engert R, Henklein P, Donini O, Zhou Q, Winkler DF, Hancock REW.

    Chem Biol    . 2006;13(10):1101-7.

  • High-throughput generation of small antibacterial peptides with improved activity.

    Hilpert K, Volkmer-Engert R, Walter T, Hancock REW.

    Nat Biotechnol    . 2005;23(8):1008-12. (Covered in Nature Drug Discovery)

 

Inhibitor/Protease Interaction

  • Complete substitutional analysis of a sunflower trypsin inhibitor with different serine proteases.

    Hilpert K    , Hansen G, Wessner H, Volkmer-Engert R, Höhne W.

    J Biochem    . 2005;138(4):383-90.
  • Unraveling sub-site specificities of peptidic serine protease inhibitors by substitutional and structural analysis.

    Höhne W, Hilpert K.

    Protein Pept Lett    . 2005;12(5):449-56.
  • Crystallization and preliminary x-ray analysis of complexes of porcine pancreatic elastase with two natural inhibitors.

    Hilpert K    , Wessner H, Scholz C, Scheerer P, Volkmer-Engert R, Krauss N.

    Protein Pept Lett    . 2004;11(4):393-9.
  • A novel method for characterizing protease/inhibitor interaction by using peptide synthesis on cellulose.

    Hilpert K    , Hansen G, Wessner H, Schneider-Mergener J, Höhne W.

    Biotechnology     2000;2:73-75,

SPOT Synthesis (Methods & Applications)

  • Cellulose-bound peptide arrays: preparation and applications.

    Hilpert K    , Winkler DF, Hancock REW.

    Biotechnol Genet Eng Rev    . 2007;24:31-106.
  • Sequence requirements and an optimization strategy for short antimicrobial peptides.

    Hilpert K    , Elliott MR, Volkmer-Engert R, Henklein P, Donini O, Zhou Q, Winkler DF, Hancock REW.

    Chem Biol    . 2006;13(10):1101-7.
  • Complete substitutional analysis of a sunflower trypsin inhibitor with different serine proteases.

    Hilpert K    , Hansen G, Wessner H, Volkmer-Engert R, Höhne W.

    J Biochem    . 2005;138(4):383-90.
  • Unraveling sub-site specificities of peptidic serine protease inhibitors by substitutional and structural analysis.

    Höhne W, Hilpert K.

    Protein Pept Lett    . 2005;12(5):449-56.
  • A novel method for characterizing protease/inhibitor interaction by using peptide synthesis on cellulose.

    Hilpert K    , Hansen G, Wessner H, Schneider-Mergener J, Höhne W.

    Biotechnology     2000;2:73-75,

 

Methods

  • A novel method for characterizing protease/inhibitor interaction by using peptide synthesis on cellulose.

    Hilpert K    , Hansen G, Wessner H, Schneider-Mergener J, Höhne W.

    Biotechnology     2000;2:73-75,

 

Reviews:

  • Antibacterial Host Defence Peptides of Bovine Origin

    Jenssen H, Hilpert K, Hancock REW.

    Journal Chimica Oggi/CHEMISTRY TODAY     2007;25(3):17-19.
  • Cellulose-bound peptide arrays: preparation and applications.

    Hilpert K    , Winkler DF, Hancock REW.

    Biotechnol Genet Eng Rev    . 2007;24:31-106.
  • Unraveling sub-site specificities of peptidic serine protease inhibitors by substitutional and structural analysis.

    Höhne W, Hilpert K.

    Protein Pept Lett    . 2005;12(5):449-56.

Inhibitor/Protease InteractionInhibitor/Protease Interaction